We use cookies to give you the best experience possible. By continuing we’ll assume you’re on board with our cookie policy

Factors That Affect the Rate of Reaction of Peroxidase Essay

Sorry, but copying text is forbidden on this website!

Purpose:

To determine the effect of various factors on the rate of reaction between an enzyme andits substrate, and also to determine the optimal ranges under which the enzyme activity ismaximized. Also to determine whether saline and alcohol are inhibitors or activators

Hypothesis:

PH factor prediction: I predict that as the pH increases so the activity of the enzyme willincrease until it reaches optimum pH range (pH 7) because the enzyme is less denaturedwhen it reaches the preferred pH level, and after this it will decrease because the activesite will change in shape and it will no longer accept substrates.Temperature factor prediction: I predict as the temperature increases, the enzymeactivities will increase because there is more energy to speed up the reaction until itreaches the optimum temperature range (room temperature which is about 20 °C), andafter that the enzyme activities will decrease because of denature of the enzymes (causechanges to active site that will no longer fit substrate)

We will write a custom essay sample on Factors That Affect the Rate of Reaction of Peroxidase specifically for you

Order now

Concentration of enzymes prediction: I predict that as the concentration of enzymeincreases, so the enzyme activities will increase because there is more enzyme to reactwith the substrates however when enzymes get saturated, the reaction will come to a plateau because eventually all the substrates will have enzymes to react with, and anyextra will have no effect on the reaction whatsoever.I predict alcohol is an inhibitor of Peroxidase because alcohol when alcohol bind to theallosteric site it changes the active site shape of the enzymes thus deactivating enzymaticactivitiesI predict salt is an activator of Peroxidase because salt contains Na ions which attaches tothe allosteric site changing the shape of the enzyme to fit a substrate.

Materials:


Peroxidase (enzyme in potato)

Hydrogen peroxide, 3%

A strong acid, pH3 (lemon juice, or HCL) 0.5

A strong base, pH 10 (drain cleaner, NaOH) 0.5 mol/L

A weak acid, pH 6 (vinegar, acetic acid( CH3COOH)) 0.5 mol/L •
A weak base, pH 8 (baking soda, sodium bicarbonate (NaHCO3)) 0.5 mol/L a

How to cite this page

Choose cite format:

Factors That Affect the Rate of Reaction of Peroxidase. (2017, Jan 13). Retrieved from http://huseyinzadealituran.com/factors-that-affect-the-rate-of-reaction-of-peroxidase-essay

We will write a custom essay sample onFactors That Affect the Rate of Reaction of Peroxidasespecifically for you

Our customer support team is available Monday-Friday 9am-5pm EST. If you contact us after hours, we'll get back to you in 24 hours or less.

By clicking "Send Message", you agree to our terms of service and privacy policy. We'll occasionally send you account related and promo emails.
No results found for “ image
Try Our service
online

Hi, I am Sara from Studymoose

Hi there, would you like to get such a paper? How about receiving a customized one? Check it out http://goo.gl/CYf83b

online

Hi, I am Sara from Studymoose

Hi there, would you like to get such a paper? How about receiving a customized one? Check it out http://goo.gl/CYf83b

image

Your Answer is very helpful for Us
Thank you a lot!

Sitemap